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Weng, Chun Hung; Tang, Jialei; Han, Kyu Young (, Journal of Physics D: Applied Physics)
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Tang, Jialei; Ren, Jinhan; Han, Kyu Young (, Nanophotonics)Abstract Fluorescence microscopy has long been a valuable tool for biological and medical imaging. Control of optical parameters such as the amplitude, phase, polarization, and propagation angle of light gives fluorescence imaging great capabilities ranging from super-resolution imaging to long-term real-time observation of living organisms. In this review, we discuss current fluorescence imaging techniques in terms of the use of tailored or structured light for the sample illumination and fluorescence detection, providing a clear overview of their working principles and capabilities.more » « less
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Lee, Jong-Chan; Ma, Ye; Han, Kyu Young; Ha, Taekjip (, ACS Photonics)
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Tang, Jialei; Han, Kyu Young (, Journal of Biophotonics)Abstract Confocal microscopy is an indispensable tool for biological imaging due to its high resolution and optical sectioning capability. However, its slow imaging speed and severe photobleaching have largely prevented further applications. Here, we present dual inclined beam line‐scanning (LS) confocal microscopy. The reduced excitation intensity of our imaging method enabled a 2‐fold longer observation time of fluorescence compared to traditional LS microscopy while maintaining a good sectioning capability and single‐molecule sensitivity. We characterized the performance of our method and applied it to subcellular imaging and three‐dimensional single‐molecule RNA imaging in mammalian cells.more » « less
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